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Zhang, Sihao; Jiang, Zhaoming; Zhang, Shuai; Xia, Mingfeng; Tian, Fang; Tian, Hu

2016-05-01

We sequenced a complete mitochondrial genome sequencing of a chronic hepatitis-associated liver cancer disease LEC rat strain for the first time. The total length of the mitogenome was 16,316 bp with 13 protein-coding genes, two ribosomal RNA genes and 22 transfer RNA genes. This mitochondrial genome sequence will provide new genetic resource into liver cancer disease.

Oxidative deamination of alicyclic primary amines by liver microsomes from rats and rabbits.

Kurebayashi, H; Tanaka, A; Yamaha, T; Tatahashi, A

1988-09-01

1. Substrate selectivity and species difference in the oxidative deamination of the alicyclic primary amines, cyclopentylamine, cyclohexylamine, cycloheptylamine, 1- and 2-aminoindane, and 1- and 2-aminotetralin were studied using liver microsomes from rats and rabbits. 2. The deamination rates of the amines were much greater with liver microsomes from rabbits than from rats . Substrate selectivity resulted in much faster deamination of 1-aminoindane and 1-aminotetralin than of the corresponding 2-amino compounds, especially in rats . 3. When 1-aminoindane and 1-aminotetralin were incubated with rat liver microsomes and NADPH under 18O2, oxygen-18 was incorporated into the deaminated products, 1-indanone and 1-tetralone. The carbinolamine is a key intermediate in the oxidative deamination by rat liver microsomes, indicating the contribution of cytochrome P-450-dependent alpha-C-oxidation to the reaction. 4. Alicyclic primary amines gave type II binding spectra with rat and rabbit liver microsomes, but the spectra appeared to contain type I components. 5. The ratios of the alcohols, cyclohexanol, 2-tetralol and 2-indanol in the deaminated products were high in both rats and rabbits. The ketones were precursors of the alcohols, and substrate selectivity in reduction of the alicyclic ketones with NADPH was similar in both species.

Low-Level Laser Irradiation Improves Functional Recovery and Nerve Regeneration in Sciatic Nerve Crush Rat Injury Model

Wang, Chau-Zen; Chen, Yi-Jen; Wang, Yan-Hsiung; Yeh, Ming-Long; Huang, Mao-Hsiung; Ho, Mei-Ling; Liang, Jen-I; Chen, Chia-Hsin

The development of noninvasive approaches to facilitate the regeneration of post-traumatic nerve injury is important for clinical rehabilitation. In this study, we investigated the effective dose of noninvasive 808-nm low-level laser therapy (LLLT) on sciatic nerve crush rat injury model. Thirty-six male Sprague Dawley rats were divided into 6 experimental groups: a normal group with or without 808-nm LLLT at 8 J/cm2 and a sciatic nerve crush injury group with or without 808-nm LLLT at 3, 8 or 15 J/cm2. Rats were given consecutive transcutaneous LLLT at the crush site and sacrificed 20 days after the crush injury. Functional assessments of nerve regeneration were analyzed using the sciatic functional index (SFI) and hindlimb range of motion (ROM). Nerve regeneration was investigated by measuring the myelin sheath thickness of the sciatic nerve using transmission electron microscopy (TEM) and by analyzing the expression of growth-associated protein 43 (GAP43) in sciatic nerve using western blot and immunofluorescence staining. We found that sciatic-injured rats that were irradiated with LLLT at both 3 and 8 J/cm2 had significantly improved SFI but that a significant improvement of ROM was only found in rats with LLLT at 8 J/cm2. Furthermore, the myelin sheath thickness and GAP43 expression levels were significantly enhanced in sciatic nerve-crushed rats receiving 808-nm LLLT at 3 and 8 J/cm2. Taken together, these results suggest that 808-nm LLLT at a low energy density (3 J/cm2 and 8 J/cm2) is capable of enhancing sciatic nerve regeneration following a crush injury. PMID:25119457

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https://www.chron.com/neighborhood/champions-klein/schools/article/Wunderlich-Intermediate-seeks-to-teach-and-feed-12849173.php

By Mayra Cruz

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Wunderlich Intermediate School partnered with Kinsmen Lutheran Church and Sustainable Harvesters to start an aquaponic garden that will grow produce as part of the school's food pantry.
Wunderlich Intermediate School partnered with Kinsmen Lutheran Church and Sustainable Harvesters to start an aquaponic garden that will grow produce as part of the school's food pantry.
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Wunderlich Intermediate School is growing produce, such as leafy greens, to feed families through the school's food pantry.
Wunderlich Intermediate School is growing produce, such as leafy greens, to feed families through the school's food pantry.
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Matthew Braud with Sustainable Harvesters partnered with Wunderlich Intermediate and Kinsmen Lutheran Church to start an aquaponic garden that will grow produce as part of the school's food pantry.
Matthew Braud with Sustainable Harvesters partnered with Wunderlich Intermediate and Kinsmen Lutheran Church to start an aquaponic garden that will grow produce as part of the school's food pantry.
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Wunderlich Intermediate School partnered with Kinsmen Lutheran Church and Sustainable Harvesters to start an aquaponic garden that will grow produce as part of the school's food pantry.
Wunderlich Intermediate School partnered with Kinsmen Lutheran Church and Sustainable Harvesters to start an aquaponic garden that will grow produce as part of the school's food pantry.
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Wunderlich Intermediate seeks to teach and feed students with aquaponic garden
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The lettuce seedlings floating on the grow beds of the aquaponic garden on Wunderlich Intermediate School are doing more than just teaching students how to grow produce.

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